Manganese-Dependent Polioviruses Caused by Mutations within the Viral Polymerase

Manganese-dependent polioviruses caused by mutations within the viral polymerase.

Viral RNA-dependent RNA polymerases exhibit great sequence diversity. Only six core amino acids are conserved across all polymerases of positive-strand RNA viruses of eukaryotes. While exploring the function of one of these completely conserved residues, asparagine 297 in the prototypic poliovirus polymerase 3D(pol), we identified three viable mutants with noncanonical amino acids at this conse...

Overexpression of DNA Polymerase Zeta Reduces the Mitochondrial Mutability Caused by Pathological Mutations in DNA Polymerase Gamma in Yeast

In yeast, DNA polymerase zeta (Rev3 and Rev7) and Rev1, involved in the error-prone translesion synthesis during replication of nuclear DNA, localize also in mitochondria. We show that overexpression of Rev3 reduced the mtDNA extended mutability caused by a subclass of pathological mutations in Mip1, the yeast mitochondrial DNA polymerase orthologous to human Pol gamma. This beneficial effect w...

A viral deubiquitylating enzyme stabilizes viral RNA- dependent RNA polymerase and affects viral infectivity

Thank you for submitting your manuscript on the identification of DUB function in the TYMV protease for consideration by The EMBO Journal. Three referees have now provided their comments, which are copied below. As you will see, the referees appreciate that your study (for the most part convincingly) establishes TYMV 98K as a bona fide new example of a viral DUB enzyme, although a few open ques...

A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity.

Selective protein degradation via the ubiquitin-proteasome system (UPS) plays an essential role in many major cellular processes, including host-pathogen interactions. We previously reported that the tightly regulated viral RNA-dependent RNA polymerase (RdRp) of the positive-strand RNA virus Turnip yellow mosaic virus (TYMV) is degraded by the UPS in infected cells, a process that affects viral...

A viral deubiquitylating enzyme targets viral RNA - dependent RNA polymerase and affects viral